Fig. 2. The relationship of glucose import and serine biosynthetic pathway through PHGDH in papillary thyroid cancer. (A) The correlation plot of glucose import and serine related pathway using Gene score in TCGA-THCA cohort. (B) Representative Western blot images of PHGDH expression in thyroid cell lines. (C) Representative Western blot images of GLUT1 expression and PHGDH expression in thyroid cell lines in relation to glucose concentrations in media. (D) Glucose uptake in TPC-1 and BCPAP cells in relation to glucose concentrations in media. (E) Serine concentrations in TPC-1 and BCPAP cells in relation to glucose concentrations in media. (F-I) Real time PCR analysis of GLUT1, PSAT1, PHGDH, and HIF-1a in TPC-1 and BCPAP cells in relation to glucose concentrations in media. These experiments are representative of more than three independent experiments. *p<0.05, **p<0.01

Int J Thyroidol 2023;16:175-83 https://doi.org/10.11106/ijt.2023.16.2.175

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